Startseite UB

Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells

Hessing, M. and van Schijndel, H. B. and van Grunsven, W. M. and Wolf, Hans J. and Middeldorp, J. M. (1992) Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells. Journal of chromatography 599 (1-2), pp. 267-272.

[img]
Preview
PDF
Download (721kB)

at PubMed


Abstract

Truncated Epstein-Barr virus (EBV) membrane antigen gp350/220 (EBV-MA) lacking the membrane anchor was expressed and secreted into the medium of recombinant Chinese hamster ovary cells that had been cultured in Plasmapur hollow-fibre modules using defined serum-free medium. The EBV-MA in the medium was concentrated by 70% (w/v) ammonium sulphate precipitation and subsequently purified by ...

plus


Export bibliographical data

Item Type:Article
Date:1992
Institutions:Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:
ValueType
1320046PubMed ID
Classification:
NotationType
AnimalsMESH
Antigens, Viral/isolation & purificationMESH
Blotting, WesternMESH
CHO CellsMESH
CricetinaeMESH
Electrophoresis, Polyacrylamide GelMESH
Enzyme-Linked Immunosorbent AssayMESH
Herpesvirus 4, Human/immunologyMESH
Recombinant Proteins/isolation & purificationMESH
Viral Envelope Proteins/isolation & purificationMESH
Viral Matrix ProteinsMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Universitätsbibliothek Regensburg
Deposited On:11 Apr 2011 10:53
Last Modified:13 Mar 2014 17:11
Item ID:20424
Owner Only: item control page
  1. University

University Library

Publication Server

Contact person
Gernot Deinzer

Telefon 0941 943-2759
Contact