Hessing, M. and van Schijndel, H. B. and van Grunsven, W. M. and Wolf, Hans J. and Middeldorp, J. M. (1992) Purification and quantification of recombinant Epstein-Barr viral glycoproteins gp350/220 from Chinese hamster ovary cells. Journal of chromatography 599 (1-2), pp. 267-272.
Truncated Epstein-Barr virus (EBV) membrane antigen gp350/220 (EBV-MA) lacking the membrane anchor was expressed and secreted into the medium of recombinant Chinese hamster ovary cells that had been cultured in Plasmapur hollow-fibre modules using defined serum-free medium. The EBV-MA in the medium was concentrated by 70% (w/v) ammonium sulphate precipitation and subsequently purified by ...
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|Institutions:||Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene|
|Subjects:||600 Technology > 610 Medical sciences Medicine|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||11 Apr 2011 10:53|
|Last Modified:||13 Mar 2014 17:11|