Browse:

The university is member of the Confederation of Open Access Repositories (COAR)

More information about Open Acces at open-acces.net

Expression of the Epstein-Barr virus major membrane proteins in Chinese hamster ovary cells

Motz, M. and Deby, G. and Jilg, Wolfgang and Wolf, Hans J. (1986) Expression of the Epstein-Barr virus major membrane proteins in Chinese hamster ovary cells. Gene 44 (2-3), pp. 353-359.

Preview

PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
1024Kb

Abstract

The gene of the major membrane antigen (gp250/350) of the Epstein-Barr virus (EBV) was isolated and inserted under the control of the SV40 early promoter into a eukaryotic expression vector, which allows selection for dhfr+ phenotype. Following transfection with this vector, Chinese hamster ovary cells express on their surfaces proteins immunologically similar to the major EBV membrane antigen. The transcript encoding gp250/350 is processed by partial splicing similarly but more efficiency than in B95-8 cells from which the DNA originates.

Item Type:

Article

Institutions:

Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene

Identification Number:

Value	Type
3023198	PubMed ID
10.1016/0378-1119(86)90202-7	DOI

Classification:

Notation	Type
Animals	MESH
Base Sequence	MESH
Cell Line	MESH
Cell Transformation, Viral	MESH
Cricetinae	MESH
Cricetulus	MESH
DNA Restriction Enzymes	MESH
Female	MESH
Genes	MESH
Genes, Viral	MESH
Genetic Vectors	MESH
Herpesvirus 4, Human/genetics	MESH
Ovary	MESH
Promoter Regions, Genetic	MESH
Transcription, Genetic	MESH
Viral Matrix Proteins	MESH
Viral Proteins/genetics	MESH