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Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA

Wu, Meng and Lin, Zhihong and Mirsky, Vladimir and Wolfbeis, Otto S. (2001) Detection of DNA hybridization with surface plasmon resonance biosensor: comparison of immobilization of oligonucleotides by ssDNA and dsDNA. In: Zhou, Yikai, (ed.) Proceedings / International Conference on Sensor Technology (ISTC 2001): 10-12 October 2001, Wuhan, China. Proc. SPIE, 4414. SPIE, the International Society for Optical Engineering, Bellingham, Wash., pp. 23-26. ISBN 0-8194-4119-8.

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Abstract

In this research, a surface-plasmon resonance biosensor for nucleic acid detection was constructed, optimized and characterized. The kinetic response on hybridization was also studied. The biosensor was use as a platform for the study of the surface coverage control by single-strand or double-strand oligonucleotide immobilization. The dsDNA immobilization and offers a new way to surface coverage ...

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Item Type:Book Section
Date:2001
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Otto S. Wolfbeis)
Identification Number:
ValueType
10.1117/12.440180 DOI
Subjects:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Owner: Petra Gürster
Deposited On:18 Jul 2011 09:41
Last Modified:18 Jul 2011 09:41
Item ID:21101
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