Rachel, Reinhard and Meyer, Carolin and Klingl, Andreas and Gürster, Sonja and Heimerl, Thomas and Wasserburger, Nadine and Burghardt, Tillmann and Küper, Ulf and Bellack, Annett and Schopf, Simone and Wirth, Reinhard and Huber, Harald and Wanner, Gerhard (2010) Analysis of the Ultrastructure of Archaea by Electron Microscopy. Methods in Cell Biology 96, pp. 47-69.
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The ultrastructural characterization of archaeal cells is done with both types of electron microscopy, transmission electron microscopy, and scanning electron microscopy. Depending on the scientific question, different preparation methods have to be employed and need to be optimized, according to the special cultivation conditions of these—in many cases extreme—microorganisms. Recent results using various electron microscopy techniques show that archaeal cells have a variety of cell appendages, used for motility as well as for establishing cell–cell and cell–surface contacts. Cryo-preparation methods, in particular high-pressure freezing and freeze-substitution, are crucial for obtaining results: (1) showing the cells in ultrathin sections in a good structural preservation, often with unusual shapes and subcellular complexity, and (2) enabling us to perform immunolocalization studies. This is an important tool to make a link between biochemical and ultrastructural studies.
|Institutions:||Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm|
|Subjects:||500 Science > 570 Life sciences|
|Created at the University of Regensburg:||Unknown|
|Deposited On:||12 Jul 2011 07:48|
|Last Modified:||12 Jul 2011 07:48|
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