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Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression.

Fath, Stephan and Bauer, Asli Petra and Liss, Michael and Spriestersbach, Anne and Maertens, Barbara and Hahn, Peter and Ludwig, Christine and Schäfer, Frank and Graf, Marcus and Wagner, Ralf (2011) Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression. PloS one 6 (3), e17596.

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Abstract

Autologous expression of recombinant human proteins in human cells for biomedical research and product development is often hampered by low expression yields limiting subsequent structural and functional analyses. Following RNA and codon optimization, 50 candidate genes representing five classes of human proteins--transcription factors, ribosomal and polymerase subunits, protein kinases, membrane ...

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Item Type:Article
Date:3 March 2011
Institutions:Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identification Number:
ValueType
8840785PubMed ID
10.1371/journal.pone.0017596DOI
Classification:
NotationType
AnimalsMESH
Base SequenceMESH
CHO CellsMESH
Codon/geneticsMESH
CricetinaeMESH
CricetulusMESH
Gene Expression RegulationMESH
Gene Knockdown TechniquesMESH
Genes, SyntheticMESH
Genetic Techniques/standardsMESH
HEK293 CellsMESH
HumansMESH
JNK Mitogen-Activated Protein Kinases/metabolismMESH
Mammals/geneticsMESH
RNA/geneticsMESH
RNA InterferenceMESH
Reference StandardsMESH
SolubilityMESH
Subjects:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Universitätsbibliothek Regensburg
Deposited On:11 Apr 2012 09:02
Last Modified:13 Mar 2014 18:45
Item ID:23753
Owner Only: item control page
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