Pawara, Kiran and Mueller, Rainer and Caioni, Massimiliano and Prang, Peter and Bogdan, Ulrich and Kunz, Werner and Weidner, Norbert (2011) Increasing capillary diameter and the incorporation of gelatin enhance axon outgrowth in alginate-based anisotropic hydrogels. Acta Biomaterialia 7 (7), pp. 2826-2834.
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Substantial recovery of function following peripheral and central nervous system (CNS) injury critically depends on longitudinally directed axon regeneration across the injury site, which requires a mech. guidance providing scaffold. We have previously shown that anisotropic alginate-based hydrogels with a defined capillary diam. (25 μm), which form via a self-organizing process driven by unidirectional diffusion of divalent cations into sodium alginate sols, promoted longitudinally oriented elongation of CNS axons in vitro and in vivo. In the present study the influence of various capillary diams. and the incorporation of gelatin to promote directed axon outgrowth and Schwann cell migration were assessed in a dorsal root ganglion outgrowth assay in vitro. Superimposing an alginate sol with Cu2+, Sr2+, or Zn2+ ion contg. solns. allowed the creation of hydrogels with capillaries 18, 25 and 55 μm in diam., resp. Axon outgrowth and Schwann cell migration were analyzed in terms of axon length/d. and Schwann cell d. within the capillary structures. Axon ingrowth into capillary hydrogels, which was always accompanied by Schwann cells, was enhanced with increasing capillary diam. The incorporation of gelatin did not influence overall axon d., but promoted the length of axon outgrowth within the hydrogels. The longitudinal orientation of axons decreased in wider capillaries, which suggests that medium-sized capillaries are the optimal substrate to elicit substantial axon growth and longitudinal orientation after axon injury.
|Institutions:||Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz|
|Keywords:||capillary diam anisotropy Manugel DJX axon outgrowth Schwann cell gelatin hydrogel dorsal root ganglion|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||09 May 2012 13:18|
|Last Modified:||09 May 2012 13:18|
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