Müller, Rainer and Ruhl, Stefan and Hiller, Karl-Anton and Schmalz, Gottfried and Schweikl, Helmut (2008) Adhesion of eukaryotic cells and Staphylococcus aureus to silicon model surfaces. Journal of Biomedical Materials Research, Part A 84A (3), pp. 817-827.
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Silicon wafers modified by silylation with different functional groups are used to study the bioactivity of surfaces with varying physicochem. properties. Oxidn. of the wafers created very hydrophilic surfaces, and moderately wettable surfaces were produced by coating with poly(ethylene glycol) (PEG). Immobilization of hydrocarbon chains to the wafers produced hydrophobic surfaces, and hydrophobicity was further increased by fluorocarbon coatings. The oxidized and the hydrocarbon-modified surfaces supported the adhesion of human MG-63 osteoblasts and 3T3 mouse fibroblasts as well as Staphylococcus aureus 8325-4. Adhesion of osteoblasts and fibroblasts, however, was decreased on highly hydrophobic fluorocarbon surfaces, whereas adhesion of S. aureus was supported. Coating of the fluorocarbon surface with fibronectin increased the no. of attached eukaryotic cells, but the accumulation of bacteria remained unchanged. In contrast, surface coatings with PEG-groups inhibited the binding of S. aureus; however, the adhesion of the eukaryotic cells was high. The no. of S. aureus on PEG-modified surfaces covered with fibronectin increased about 2-fold, yet it was still decreased to 25-30% related to the no. of bacteria on other surfaces. These findings provide evidence that the PEG-modified surfaces showed selective bioactivity, preventing the attachment of a microbial pathogen but supporting the adhesion of eukaryotic cells.
|Institutions:||Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz|
|Keywords:||silicon silylation surface wettability protein coating bacteria cell adhesion|
|Subjects:||500 Science > 540 Chemistry & allied sciences|
|Refereed:||Yes, this version has been refereed|
|Created at the University of Regensburg:||Yes|
|Deposited On:||18 Jun 2012 07:08|
|Last Modified:||18 Jun 2012 07:08|
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