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High throughput quantification of cholesterol and cholesteryl ester by electrospray ionization tandem mass spectrometry (ESI-MS/MS)

DOI to cite this document:
10.5283/epub.1020
Liebisch, Gerhard ; Binder, Marion ; Schifferer, Rainer ; Langmann, Thomas ; Schulz, Berta ; Schmitz, Gerd
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Date of publication of this fulltext: 05 Aug 2009 13:25


Abstract

Analysis of free cholesterol (FC) is not well suited for electrospray ionization (ESI); however, cholesteryl ester (CE) form ammonium adducts in positive ion mode and generate a fragment ion of m/z 369 upon collision-induced fragmentation. In order to allow parallel analysis of FC and CE using ESI tandem mass spectrometry (ESI-MS/MS), we developed an acetyl chloride derivatization method to ...

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