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Substrate preferences of Vsr DNA mismatch endonuclease and their consequences for the evolution of the Escherichia coli K-12 genome

Gläsner, W., Merkl, Rainer, Schellenberger, V. and Fritz, Hans Joachim (1995) Substrate preferences of Vsr DNA mismatch endonuclease and their consequences for the evolution of the Escherichia coli K-12 genome. Journal of Molecular Biology 245 (1), pp. 1-7.

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Other URL: http://linkinghub.elsevier.com/retrieve/pii/S0022283684700015


Abstract

The substrate spectrum of Vsr DNA mismatch endonuclease of Escherichia coli K-12 was investigated using fluorescence-labelled oligonucleotide substrates and a DNA sequencer for detection and quantification of substrates and reaction products. Fourteen substrates were found to be processed by the enzyme, which differ in one or two positions from the canonical pentanucleotide sequence CTA/TGG (T ...

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Item type:Article
Date:1995
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl
Identification Number:
ValueType
7823316PubMed ID
Classification:
NotationType
Base SequenceMESH
DNA Repair/geneticsMESH
Deoxyribonuclease I/genetics*MESH
Deoxyribonuclease I/metabolismMESH
Escherichia coli/enzymologyMESH
Escherichia coli/genetics*MESH
EvolutionMESH
Fluorescent DyesMESH
Genome, Bacterial*MESH
Molecular Sequence DataMESH
MutagenesisMESH
Oligonucleotides/analysisMESH
Substrate Specificity/geneticsMESH
Keywords:Base Sequence DNA Repair/genetics Deoxyribonuclease I/*genetics/metabolism Escherichia coli/enzymology/*genetics Evolution Fluorescent Dyes *Genome, Bacterial Molecular Sequence Data Mutagenesis Oligonucleotides/analysis Substrate Specificity/genetics Support, Non-U.S. Gov't
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:No
Item ID:10910
Owner only: item control page
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