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Fast quantitative assay of sequence-specific endonuclease activity based on DNA sequencer technology

Gläsner, W. ; Merkl, Rainer ; Schmidt, Sebastian ; Cech, D. ; Fritz, Hans Joachim



Abstract

A quantitative assay of the sequence-specific endonuclease activity of Vsr DNA mismatch endonuclease is described. The procedure rests on fluorescently labelled oligonucleotide substrates and an automated DNA sequencer to determine amounts of both educt and product of the reaction; thus each individual measurement is internally standardized. The assay achieves high sample throughput by parallel ...

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