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Chorismate mutase of Thermus thermophilus is a monofunctional AroH class enzyme inhibited by tyrosine

Helmstaedt, Kerstin ; Heinrich, Gabriele ; Merkl, Rainer ; Braus, Gerhard H.


aroG, encoding the monofunctional chorismate mutase (TtCM) of the thermophilic gram-negative bacterium Thermus thermophilus, was cloned and its gene product characterized. TtCM was purified to homogeneity on an SDS polyacrylamide gel as a His-fusion protein with a deduced molecular mass of 15.8 kDa. The enzyme belongs to the rare group of AroH-type chorismate mutases which are mainly found in ...


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