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Versatile controlling system for cryopreparation techniques in electron microscopy

Humbel, B. M. ; Weber, K. ; Merkl, Rainer



Abstract

A new setup for freeze-substitution and a versatile controlling system has been developed. Our goal was to build a simple system allowing precise control of the physical parameters of freeze-substitution experiments to learn more about their influences on the cellular ultrastructure and immunoreactivity of macromolecules. An improved apparatus for freeze-substitution, based on liquid nitrogen ...

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