Transcription In Vivo and In Vitro of the Histone-Encoding Gene hmfB from the Hyperthermophilic Archaeon Methanothermus fervidus - Publikationsserver der Universität Regensburg

URN zum Zitieren dieses Dokuments:: urn:nbn:de:bvb:355-epub-109618

Thomm, Michael ; Sandman, K. ; Frey, G. ; Koller, G. ; Reeve, J.

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Veröffentlichungsdatum dieses Volltextes: 17 Nov 2009 10:26

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Zusammenfassung

Immediately upstream of the hmJB gene, in a DNA fragment cloned from Methanothermusfervidus, are two identical tandemly repeated copies of a 73-bp sequence that contain the sequence 5'TTTATATA, which conforms precisely to the consensus TATA box element proposed for methanogen promoters. By using this duplicated region as the template DNA and a cell-free transcription system derived from ...