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Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein

Hecht, S. ; Eisenreich, W. ; Adam, P. ; Amslinger, S. ; Kis, K. ; Bacher, A. ; Arigoni, D. ; Rohdich, F.



Abstract

Recombinant Escherichia coli cells engineered for the expression of the xyIB gene in conjunction with genes of the nonmevalonate pathway were supplied with C-13-labeled 1-deoxy-D-xylulose. Cell extracts were analyzed directly by NMR spectroscopy. C-13-labeled 2C-methyl-D-erythritol 2,4-cyclodiphosphate was detected at high levels in cells expressing xyIB, ispC, ispD, ispE, and ispF. The ...

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