Rohdich, F. ; Hecht, S. ; Gärtner, K. ; Adam, P. ; Krieger, C. ; Amslinger, S. ; Arigoni, D. ; Bacher, A. ; Eisenreich, W.
Alternative Links zum Volltext:WebofscienceDOI
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Proc. Natl. Acad. Sci. USA |
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| Verlag: | National Academy of Sciences |
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| Band: | 99 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 3 |
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| Seitenbereich: | S. 1158-1163 |
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| Datum: | 2002 |
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| Zusätzliche Informationen (Öffentlich): | Times Cited: 94 |
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| Institutionen: | Chemie und Pharmazie > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger |
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| Identifikationsnummer: | | Wert | Typ |
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| ISI:000173752500014 | Web of Science | | 10.1073/pnas.032658999 | DOI |
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| Stichwörter / Keywords: | biosynthesis of isoprenoids
1-hydroxy-2-methyl-2-(E)-butenyl
4-diphosphate isopentenyl diphosphate
dimethylallyl diphosphate
Isopentenyl-diphosphate isomerase
escherichia-coli
isoprenoid
biosynthesis
2-c-methyl-d-erythritol 4-phosphate
4-(cytidine
5'-diphospho)-2-c-methyl-d-erythritol
5-phosphate
precursor
thiamin
enzyme
gene |
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| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 540 Chemie |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Nein |
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| Dokumenten-ID: | 11077 |
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Zusammenfassung
Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with ...
Zusammenfassung
Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-C-13(5)]1-deoxy-D-xylulose into a 5:1 mixture of [U-C-13(5)]isopentenyl diphosphate and [U-C-13(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.
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