Soluble Interleukin-2 Receptors Inhibit Interleukin 2-Dependent Proliferation and Cytotoxicity: Explanation for Diminished Natural Killer Cell Activity in Cutaneous T-Cell Lymphomas In Vivo?

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urn:nbn:de:bvb:355-epub-112064 Copy

Dummer, Reinhard ; Posseckert, Gerhard ; Nestle, Frank ; Witzgall, Ralph ; Burger, Mathias ; Becker, Jürgen C. ; Schäfer, Erwin ; Wiede, Johannes ; Sebald, Walter ; Burg, Günter

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Date of publication of this fulltext: 06 Dec 2010 09:14

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Item type:	Article
Journal or Publication Title:	Journal of Investigative Dermatology
Publisher:	Blackwell Publishing
Volume:	98
Number of Issue or Book Chapter:	1
Page Range:	pp. 50-54
Date:	1992
Institutions:	Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall
Identification Number:	Value Type 10.1111/1523-1747.ep12494223 DOI
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	No
Item ID:	11206

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Abstract

In patients with cutaneous T-cell lymphomas (CTCL), soluble interleukin-2 receptor serum levels (sIL-2R) were determined by ELISA technique, and natural killer cell (NK) activity, by a 4-h chromium-51 release assay. Decrease of NK activity correlated with the augmentation of serum sIL-2R. After a 4-d stimulation with interleukin 2 CTCL patients' peripheral mononuclear cells (PMC) showed an ...

Abstract

In patients with cutaneous T-cell lymphomas (CTCL), soluble interleukin-2 receptor serum levels (sIL-2R) were determined by ELISA technique, and natural killer cell (NK) activity, by a 4-h chromium-51 release assay. Decrease of NK activity correlated with the augmentation of serum sIL-2R. After a 4-d stimulation with interleukin 2 CTCL patients' peripheral mononuclear cells (PMC) showed an increase of cytotoxic activity similar to that in healthy donors' PMC. Normal donors' PMC demonstrated a diminished IL-2-induced cytotoxic activity in 25% CTCL serum (sIL-2R of 3000, 7330, and 10700 U/mI, respectively) compared to control serum (sIL-2R of 400, 340, and 420 U/ml, respectively). IL-2-dependent proliferation of 2-d phytohemagglutinin (PHA) blasts was lower in CTCL serum than in control serum. sIL-2R was enriched from one CTCL patient's serum by IL-2 affinity chromatography. Transfection of the Tac gene into NIH/3T3 fibroblasts resulted in the production of a recombinant sIL-2R. The presence Of enriched native or recombinant slL-2R inhibited interleukin-2-dependent generation of cytotoxic activity and PHA blast proliferation. We suggest that elevated sIL- 2R levels account for diminished NK activity by neutralizing iriterleukin 2 in CTCL patients.

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