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Radiochemical and luminescence-based binding and functional assays for human histamine receptors using genetically engineered cells

Mosandl, Johannes (2009) Radiochemical and luminescence-based binding and functional assays for human histamine receptors using genetically engineered cells. PhD, Universität Regensburg.

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Date of publication of this fulltext: 18 Jan 2010 14:09

Abstract (English)

For the discovery and detailed pharmacological characterisation of new ligands of G-protein coupled receptors simple, robust and reliable in vitro assays using whole cells are required. This thesis aimed at the development of new binding and functional assays for the human histamine H1, H2 and H4 receptor (hH1R, hH2R, hH4R), respectively. Whereas the ratiometric fura-2 based calcium assay in ...

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Translation of the abstract (German)

Zur Identifizierung und vertieften pharmakologischen Charakterisierung neuer Liganden G-Protein gekoppelter Rezeptoren werden zelluläre In-vitro-Testsysteme (Assays) benötigt, die sich durch leichte Durchführbarkeit, Robustheit und Verlässlichkeit auszeichnen. Die vorliegende Arbeit hatte die Entwicklung neuer Bindungs- und Aktivitätsassays für die humanen Histamin Rezeptoren H1, H2 und H4 (hH1R, ...

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Item type:Thesis of the University of Regensburg (PhD)
Date:8 October 2009
Referee:Prof. Dr. Armin Buschauer
Date of exam:7 August 2009
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Keywords:Calcium , Durchflusscytometrie , Fluoreszenz , Radioaktivität , Aequorin , CRE , Histaminrezeptor , Ligand <Biochemie> , Biologische Aktivität , G-Protein gekoppelter Rezeptor , chimäres G-Protein , Fura-2 , pharmakologische In-vitro-Assays , HEK293 Zellen , G-protein coupled receptor , chimeric G-protein , fura-2 , pharmacological in vitro assays , HEK293 cells
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:12335
Owner only: item control page

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