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Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering

Lehle, Karla, Hoenika, Markus, Jacobs, Volker R., Schmid, Franz X. and Birnbaum, Dietrich E. (2006) Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering. Tissue engineering 12 (12), pp. 3439-3447.

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Date of publication of this fulltext: 05 Aug 2009 13:27

Other URL: http://www.liebertonline.com/doi/pdf/10.1089/ten.2006.12.3439


Abstract

We analyzed a cryopreservation protocol which improves long-term storage of endothelial cells (EC) for tissue engineering purposes. Human umbilical vein EC were frozen in a high-potassium solution containing 10% dimethyl sulfoxide using 3 different cooling rates. After a storage time in liquid nitrogen of 1, 4, or 12 months, samples were thawed and compared to fresh cells in terms of growth ...

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Item type:Article
Date:December 2006
Institutions:Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Unknown
Item ID:1292
Owner only: item control page

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