Identification and Reduction of Cryoinjury in Endothelial Cells: A First Step toward Establishing a Cell Bank for Vascular Tissue Engineering

DOI to cite this document:: 10.5283/epub.1292

Lehle, Karla ; Hoenika, Markus ; Jacobs, Volker R. ; Schmid, Franz X. ; Birnbaum, Dietrich E.

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Date of publication of this fulltext: 05 Aug 2009 13:27

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Item type:	Article
Journal or Publication Title:	Tissue engineering
Volume:	12
Number of Issue or Book Chapter:	12
Page Range:	pp. 3439-3447
Date:	December 2006
Institutions:	Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Unknown
Item ID:	1292

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Abstract

We analyzed a cryopreservation protocol which improves long-term storage of endothelial cells (EC) for tissue engineering purposes. Human umbilical vein EC were frozen in a high-potassium solution containing 10% dimethyl sulfoxide using 3 different cooling rates. After a storage time in liquid nitrogen of 1, 4, or 12 months, samples were thawed and compared to fresh cells in terms of growth ...

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