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Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways.

Jürgens, Catharina, Strom, A., Wegener, D., Hettwer, Stefan, Wilmanns, Matthias and Sterner, Reinhard (2000) Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways. Proceedings of the National Academy of Sciences of the United States of America 97 (18), pp. 9925-30.

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Abstract

Enzymes participating in different metabolic pathways often have similar catalytic mechanisms and structures, suggesting their evolution from a common ancestral precursor enzyme. We sought to create a precursor-like enzyme for N'-[(5'-phosphoribosyl)formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (ProFAR) isomerase (HisA; EC ) and phosphoribosylanthranilate (PRA) isomerase (TrpF; EC ), ...

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Item type:Article
Date:2000
Institutions:Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner
Identification Number:
ValueType
10195898PubMed ID
10.1073/pnas.160255397DOI
Classification:
NotationType
Aldose-Ketose Isomerases/metabolismMESH
Amino Acid SequenceMESH
Amino Acid SubstitutionMESH
CatalysisMESH
Cloning, MolecularMESH
Directed Molecular EvolutionMESH
Escherichia coli/geneticsMESH
Evolution, MolecularMESH
Gene LibraryMESH
KineticsMESH
Molecular Sequence DataMESH
MutagenesisMESH
PlasmidsMESH
Protein Structure, SecondaryMESH
Recombinant Proteins/metabolismMESH
Sequence AlignmentMESH
Dewey Decimal Classification:500 Science > 570 Life sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:13703
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