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Large-scale production of human tumorcytotoxic macrophages grown from blood monocytes of cancer patients

Brugger, W. ; Scheibenbogen, C. ; Krause, S. ; Andreesen, Reinhard


For adoptive immunotherapy protocols using cells of the macrophage (M phi) system, well differentiated and functionally competent effector cells are required. In this presentation the generation of a large number of M phi grown in vitro from blood monocytes (mo) is reported. Mononuclear cells (MNC) were collected by cytapheresis and subsequent Ficoll centrifugation. Mean yield was 6.9 x 10(9) MNC ...


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