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An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity

Fischer, Karin ; Andreesen, Reinhard ; Mackensen, Andreas



Abstract

The cytotoxic activity of T lymphocytes, natural killer and lymphokine-activated killer cells is usually tested by radioactive assays, which detect the release of cytoplasmic contents after plasma membrane disintegration of dying cells. In contrast to this indirect evaluation of cytotoxicity, we describe here an improved fluorescence assay that is based on the direct quantitative and qualitative ...

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