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An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity

Fischer, Karin, Andreesen, Reinhard and Mackensen, Andreas (2002) An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity. Journal of immunological methods 259 (1-2), pp. 159-69.

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The cytotoxic activity of T lymphocytes, natural killer and lymphokine-activated killer cells is usually tested by radioactive assays, which detect the release of cytoplasmic contents after plasma membrane disintegration of dying cells. In contrast to this indirect evaluation of cytotoxicity, we describe here an improved fluorescence assay that is based on the direct quantitative and qualitative ...


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Item type:Article
Institutions:Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie)
Identification Number:
11730851PubMed ID
Annexin A5MESH
Cell Death/immunologyMESH
Cytotoxicity, ImmunologicMESH
Flow Cytometry/methodsMESH
Fluorescent DyesMESH
Organic ChemicalsMESH
Sensitivity and SpecificityMESH
T-Lymphocytes, Cytotoxic/immunologyMESH
Tumor Cells, CulturedMESH
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:14358
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