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Analysis of the immune response against tetanus toxoid: enumeration of specific T helper cells by the Elispot assay

Mayer, Stephanie ; Laumer, Monika ; Mackensen, Andreas ; Andreesen, Reinhard ; Krause, Stefan W


Tetanus toxoid (TT) is an antigen known to induce strong T cell specific immune responses in humans after vaccination. Here we have used the Elispot assay to assess the number of TT-specific Interferon-gamma (IFN-gamma) secreting T cells present in individuals and monitored the number of TT specific T cells present in the donors for more than two years. In each of the 22 healthy volunteers ...


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