van Oers, Johanna M. M., Wild, Peter J., Burger, Maximilian, Denzinger, Stefan, Stoehr, Robert, Rosskopf, Elke, Hofstaedter, Ferdinand, Steyerberg, Ewout W., Klinkhammer-Schalke, Monika, Zwarthoff, Ellen C., van der Kwast, Theodorus H. and Hartmann, Arndt
(2007)
FGFR3 mutations and a normal CK20 staining pattern define low-grade noninvasive urothelial bladder tumours.
European urology 52 (3), pp. 760-768.
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Abstract
OBJECTIVES: Molecular markers superior to conventional clinicopathologic parameters are needed to predict disease courses in bladder cancer patients. In this study, we investigated four markers (Ki-67, TP53, CK20, FGFR3) in primary urothelial bladder tumours and compared them with traditional pathologic features. METHODS: Tissue microarrays were used to analyse CK20, TP53, and Ki-67 expression ...
Abstract
OBJECTIVES: Molecular markers superior to conventional clinicopathologic parameters are needed to predict disease courses in bladder cancer patients. In this study, we investigated four markers (Ki-67, TP53, CK20, FGFR3) in primary urothelial bladder tumours and compared them with traditional pathologic features. METHODS: Tissue microarrays were used to analyse CK20, TP53, and Ki-67 expression immunohistochemically in 255 unselected patients. FGFR3 mutations were detected by SNaPshot analysis. RESULTS: Abnormal CK20 expression was strongly associated with higher tumour grades and stages (p < 0.001); however, 65% of pTa tumours revealed an abnormal CK20 pattern. In the group of pTaG1 tumours, 59% presented with an abnormal CK20 pattern, whereas 82% carried the FGFR3 mutation. In the group of bladder tumours with normal CK20 pattern, the FGFR3 gene was mutated in 89%, whereas a mutated FGFR3 gene was found in only 37% of cases with abnormal CK20 expression (p < 0.001). All markers proved to be strong predictors of disease-specific survival in univariate studies. However, in multivariate analyses they were not independent from classical pathologic parameters. None of the molecular markers was significantly associated with tumour recurrence. CONCLUSIONS: Dysregulation of CK20 expression is an early event in the carcinogenesis of papillary noninvasive bladder cancer, but occurs later than FGFR3 mutations. The group of low-grade noninvasive papillary tumours is defined by the presence of an FGFR3 mutation and a normal CK20 expression pattern.
Export bibliographical data
| Item type: | Article |
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| Date: | 2007 |
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| Institutions: | Medicine > Lehrstuhl für Pathologie |
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| Identification Number: | | Value | Type |
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| 17240042 | PubMed ID | | 10.1016/j.eururo.2007.01.009 | DOI |
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| Classification: | | Notation | Type |
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| Aged | MESH | | Carcinoma, Papillary/pathology | MESH | | DNA, Neoplasm/genetics | MESH | | Female | MESH | | Follow-Up Studies | MESH | | Gene Expression Regulation, Neoplastic | MESH | | Humans | MESH | | Immunohistochemistry | MESH | | Keratin-20/metabolism | MESH | | Ki-67 Antigen/metabolism | MESH | | Male | MESH | | Mutation | MESH | | Neoplasm Staging | MESH | | Polymerase Chain Reaction | MESH | | Receptor, Fibroblast Growth Factor, Type 3/metabolism | MESH | | Retrospective Studies | MESH | | Time Factors | MESH | | Tumor Markers, Biological/metabolism | MESH | | Tumor Suppressor Protein p53/metabolism | MESH | | Urinary Bladder Neoplasms/pathology | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Unknown |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 14596 |
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