Schwinzer, R., Sommermeyer, H., Schlitt, Hans-Jürgen, Schmidt, R. E. and Wonigeit, K.
(1991)
Activation of human thymocytes by antibodies to the CD3/T-cell receptor complex: triggering of different epitopes results in different signals.
Cellular immunology 136 (2), pp. 318-328.
Date of publication of this fulltext: 10 May 2010 12:20at PubMed
at publisher (via DOI)
Abstract
Monoclonal antibodies (mAb) against the CD3/T cell receptor (TcR) complex were analyzed for their ability to activate human thymocytes. In addition to mAb detecting epitopes on the CD3 complex (OKT3, BMA 030) the activation potential of recently developed mAb against common epitopes on the alpha/beta T-cell receptor (anti-TcR mAb: BMA 031, BMA 032) was evaluated. Several differences were observed ...
Abstract
Monoclonal antibodies (mAb) against the CD3/T cell receptor (TcR) complex were analyzed for their ability to activate human thymocytes. In addition to mAb detecting epitopes on the CD3 complex (OKT3, BMA 030) the activation potential of recently developed mAb against common epitopes on the alpha/beta T-cell receptor (anti-TcR mAb: BMA 031, BMA 032) was evaluated. Several differences were observed between the two types of mAb: (a) Binding of the tested anti-CD3 mAb to thymocytes resulted in a rapid increase in the level of cytoplasmic free calcium ions [Ca2+]i, whereas no significant changes in [Ca2+]i were detected in thymocytes stimulated with BMA 031 or BMA 032. (b) Induction of effective proliferation induced by mAb OKT3 depended on exogenous IL-2 and in addition on the presence of accessory cells or phorbol-ester. Proliferation induced by BMA 031 only required exogenous IL-2. (c) OKT3 but not BMA 031 inhibited proliferation of thymocytes induced via the CD2 molecule. These studies indicate that anti-CD3 and anti-TcR mAb transduce different signals in thymocytes. Since the two types of mAb are directed to the same molecular complex the observed differences also support the idea that there are functionally different compartments in the CD3/TcR complex which may activate different signaling pathways.
Export bibliographical data
| Item type: | Article |
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| Date: | September 1991 |
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| Institutions: | Medicine > Lehrstuhl für Chirurgie |
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| Identification Number: | | Value | Type |
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| 1714794 | PubMed ID | | 10.1016/0008-8749(91)90355-F | DOI |
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| Classification: | | Notation | Type |
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| Antibodies, Monoclonal/immunology | MESH | | Antigens, CD/immunology | MESH | | Antigens, CD2 | MESH | | Antigens, CD3 | MESH | | Antigens, Differentiation, T-Lymphocyte/immunology | MESH | | Calcium/physiology | MESH | | Epitopes | MESH | | Flow Cytometry | MESH | | Humans | MESH | | Lymphocyte Activation | MESH | | Receptors, Antigen, T-Cell/physiology | MESH | | Receptors, Immunologic/immunology | MESH | | Signal Transduction | MESH | | T-Lymphocytes/immunology | MESH | | Thymus Gland/cytology | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Unknown |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 14723 |
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