| Dokumentenart: | Artikel | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Titel eines Journals oder einer Zeitschrift: | Journal of biomolecular screening | ||||||||||||||||||
| Verlag: | SAGE PUBLICATIONS INC | ||||||||||||||||||
| Ort der Veröffentlichung: | THOUSAND OAKS | ||||||||||||||||||
| Band: | 9 | ||||||||||||||||||
| Nummer des Zeitschriftenheftes oder des Kapitels: | 4 | ||||||||||||||||||
| Seitenbereich: | S. 273-285 | ||||||||||||||||||
| Datum: | 2004 | ||||||||||||||||||
| Institutionen: | Medizin > Lehrstuhl für Pathologie | ||||||||||||||||||
| Identifikationsnummer: |
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| Klassifikation: |
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| Stichwörter / Keywords: | 3-DIMENSIONAL CELL-CULTURES; HUMAN GLIOMA SPHEROIDS; HUMAN-TUMOR SPHEROIDS; EARLY CLINICAL-TRIALS; IN-VITRO; PHOTODYNAMIC THERAPY; MONOCLONAL-ANTIBODY; MATHEMATICAL-MODEL; DIFFERENT REGIONS; EXTRAVASCULAR TRANSPORT; cell-based assay; 3-D culture; spheroid; co-culture; anti-tumor drug testing | ||||||||||||||||||
| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin | ||||||||||||||||||
| Status: | Veröffentlicht | ||||||||||||||||||
| Begutachtet: | Unbekannt / Keine Angabe | ||||||||||||||||||
| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe | ||||||||||||||||||
| Dokumenten-ID: | 15508 |
Web of ScienceZusammenfassung
Over the past few years, establishment and adaptation of cell-based assays for drug development and testing has become an important topic in high-throughput screening (HTS). Most new assays are designed to rapidly detect specific cellular effects reflecting action at various targets. However, although more complex than cell-free biochemical test systems, HTS assays using monolayer or suspension ...
Zusammenfassung
Over the past few years, establishment and adaptation of cell-based assays for drug development and testing has become an important topic in high-throughput screening (HTS). Most new assays are designed to rapidly detect specific cellular effects reflecting action at various targets. However, although more complex than cell-free biochemical test systems, HTS assays using monolayer or suspension cultures still reflect a highly artificial cellular environment and may thus have limited predictive value for the clinical efficacy of a compound. Today's strategies for drug discovery and development, be they hypothesis free or mechanism based, require facile, HTS-amenable test systems that mimic the human tissue environment with increasing accuracy in order to optimize preclinical and preanimal selection of the most active molecules from a large pool of potential effectors, for example, against solid tumors. Indeed, it is recognized that 3-dimensional cell culture systems better reflect the in vivo behavior of most cell types. However, these 3-D test systems have not yet been incorporated into mainstream drug development operations. This article addresses the relevance and potential of 3-D in vitro systems for drug development, with a focus on screening for novel antitumor drugs. Examples of 3-D cell models used in cancer research are given, and the advantages and limitations of these systems of intermediate complexity are discussed in comparison with both 2-D culture and in vivo models. The most commonly used 3-D cell culture systems, multicellular spheroids, are emphasized due to their advantages and potential for rapid development as HTS systems. Thus, multicellular tumor spheroids are an ideal basis for the next step in creating HTS assays, which are predictive of in vivo antitumor efficacy.
Metadaten zuletzt geändert: 29 Sep 2021 07:37
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