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Phosphorylation of the sarcoplasmic calcium-activated adenosinetriphosphatase as studied by ³¹P nuclear magnetic resonance

Sontheimer, G. M. ; Kalbitzer, Hans Robert ; Hasselbach, W.


A reinvestigation of a study of Fossel et al. [Fossel, E. T., Post, R. L., O'Hara, D.S., & Smith, T. W. (1981) Biochemistry 20, 7215-7219] in which the 31P nuclear magnetic resonance (NMR) signal of the phosphointermediate of the sarcoplasmic (Ca2+, Mg2+)-ATPase has been identified shows that the signal they describe most probably originates from free Mg . ATP but not from the phosphoenzyme ...


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