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Combined chemical shift changes and amino acid specific chemical shift mapping of protein-protein interactions

Schumann, Frank H. ; Riepl, Hubert ; Maurer, Till ; Gronwald, Wolfram ; Neidig, Klaus-Peter ; Kalbitzer, Hans Robert



Abstract

Protein-protein interactions are often studied by chemical shift mapping using solution NMR spectroscopy. When heteronuclear data are available the interaction interface is usually predicted by combining the chemical shift changes of different nuclei to a single quantity, the combined chemical shift perturbation Deltadelta comb In this paper different procedures (published and non-published) to ...

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