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DNA Staining in Agarose Gels with Zn2+ -Cyclen-Pyrene

Schmidt, Florian, Schmidt, J., Riechers, A., Haase, S., Bosserhoff, Anja-Katrin, Heilmann, J. and König, Burkhard (2010) DNA Staining in Agarose Gels with Zn2+ -Cyclen-Pyrene. Nucleosides, Nucleotides & Nucleic Acids (29), pp. 748-759.

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A pyrene-labeled Zn²+-cyclen complex for the staining of DNA in agarose gels is reported. The metal chelate coordinates reversibly to the DNA phosphate backbone, which induces the formation of pyrene excimers. The typical pyrene excimer emission is used for the detection of the DNA. Staining is limited to agarose gels and is less sensitive than ethidium bromide, but DNA amounts as low as 10 ng ...


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Item type:Article
Institutions:Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König
Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann)
Medicine > Lehrstuhl für Pathologie
Identification Number:
20924956PubMed ID
Keywords:DNA staining; Zn2+-cyclen; pyrene; excimer emission
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:16993
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