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| Dokumentenart: | Artikel | ||||
|---|---|---|---|---|---|
| Titel eines Journals oder einer Zeitschrift: | Pharmaceutical research | ||||
| Verlag: | Springer | ||||
| Band: | 19 | ||||
| Nummer des Zeitschriftenheftes oder des Kapitels: | 8 | ||||
| Seitenbereich: | S. 1236-1243 | ||||
| Datum: | 2002 | ||||
| Zusätzliche Informationen (Öffentlich): | CAN 138:265054 1-1 Pharmacology 83869-56-1 (GM-CSF) Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mRNA, response; relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.); 16561-29-8 (Phorbol 12-myristate 13-acetate) Role: ARG (Analytical reagent use), ANST (Analytical study), USES (Uses) (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.); 404-86-4 (Capsaicin); 548-04-9 (Hypericin); 7295-85-4 ((+-)-Catechin); 59865-13-3 (Cyclosporin A) Role: ANT (Analyte), PAC (Pharmacological activity), THU (Therapeutic use), ANST (Analytical study), BIOL (Biological study), USES (Uses) (validation of method; relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) | ||||
| Institutionen: | Chemie und Pharmazie > Institut für Pharmazie > Lehrstuhl Pharmazeutische Biologie (Prof. Heilmann) | ||||
| Identifikationsnummer: |
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| Stichwörter / Keywords: | Cyclins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (D1, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Transcription factors Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (IkB-alpha (NF-kB inhibitor alpha ), mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Animal cell line (JURKAT relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Transcription factors Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (NFAT2 (nuclear factor of activated T-cell, 2), mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) PCR (RT-PCR (reverse transcription-PCR), real-time relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Fas antigen Interleukin 2 Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Proteins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (p65, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Anti-inflammatory agents Cytotoxic agents Drug screening Human (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) mRNA Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti- Actins Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (beta -, mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) Interferons Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (gamma , mRNA, response relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR) and new possibilities for the screening of anti-inflammatory and cytotoxic compds.) antiinflammatory cytotoxic agent screening mRNA real time RT PCR | ||||
| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 500 Naturwissenschaften und Mathematik > 540 Chemie | ||||
| Status: | Veröffentlicht | ||||
| Begutachtet: | Ja, diese Version wurde begutachtet | ||||
| An der Universität Regensburg entstanden: | Nein | ||||
| Dokumenten-ID: | 17202 |
Zusammenfassung
The objective of this study was quantification of the pro-inflammatory action of mitogens on mRNA levels of growth-related genes, transcription factors, and cytokines in T cells as markers for the screening of compds. with immunomodulatory, anti-inflammatory or cytotoxic potential. A reverse transcription-real time-polymerase chain reaction assay with TaqMan probes was developed. Jurkat T cells ...
Zusammenfassung
The objective of this study was quantification of the pro-inflammatory action of mitogens on mRNA levels of growth-related genes, transcription factors, and cytokines in T cells as markers for the screening of compds. with immunomodulatory, anti-inflammatory or cytotoxic potential. A reverse transcription-real time-polymerase chain reaction assay with TaqMan probes was developed. Jurkat T cells were treated with cyclosporin A, hypericin, capsaicin, and catechin before phorbol 12-myristate 13-acetate stimulation, and their effects on the relative mRNA levels were detd. A cell viability assay was performed in parallel. Cyclosporin A and capsaicin were potent inhibitors of PMA-induced cytokine transcription. Cyclosporin A further targeted cyclin D1 transcription. Capsaicin exhibited no effects on the cell viability at low concns., whereas cyclosporin A did. Hypericin down-regulated nearly all investigated mRNAs, resulting in a strong time-dependent cytotoxicity. Catechin showed no effects on mRNA levels and cell viability. Thus, the inhibition of the up-regulation of mRNA levels of cytokines points to a specific anti-inflammatory potential of capsaicin. Hypericin showed no specific effects on the mRNA expression. The overall decrease of mRNA levels is probably an early indication of the strong cytotoxic effect obsd. after 48 h. Therefore, quantification of mRNA levels by reverse transcription-real time-polymerase chain reaction is, in combination with the monitoring of cell viability, a valuable tool to distinguish between specific immunomodulatory and cytotoxic effects in vitro.
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