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| Dokumentenart: | Artikel | ||||
|---|---|---|---|---|---|
| Titel eines Journals oder einer Zeitschrift: | Bioorganic & medicinal chemistry | ||||
| Verlag: | Elsevier | ||||
| Band: | 11 | ||||
| Nummer des Zeitschriftenheftes oder des Kapitels: | 6 | ||||
| Seitenbereich: | S. 1057-1063 | ||||
| Datum: | 2003 | ||||
| Zusätzliche Informationen (Öffentlich): | CAN 139:95045 1-7 Pharmacology 10102-43-9 (Nitric oxide); 83869-56-1 (Granulocyte-macrophage colony-stimulating factor) Role: BSU (Biological study, unclassified), BIOL (Biological study) (curcumin differentially modulates mRNA profiles in Jurkat T and human peripheral blood mononuclear cells); 458-37-7 (Curcumin) Role: PAC (Pharmacological activity), THU (Therapeutic use), BIOL (Biological study), USES (Uses) (curcumin differentially modulates mRNA profiles in Jurkat T and human peripheral blood mononuclear cells) | ||||
| Institutionen: | Chemie und Pharmazie > Institut für Pharmazie > Lehrstuhl Pharmazeutische Biologie (Prof. Heilmann) | ||||
| Identifikationsnummer: |
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| Stichwörter / Keywords: | Cyclins Role: BSU (Biological study, unclassified), BIOL (Biological study) (D1 curcumin differentially modulates mRNA profiles in Jurkat T and human peripheral blood mononuclear cells) Antitumor agents CD4-positive T cell Human Mononuclear cell (curcumin differentially modulates mRNA profiles in Jurkat T and human peripheral blood mononuclear cells) Gene Interleukin 2 Interleukin 6 mRNA Role: BSU (Biological study, unclassified), BIOL (Biological study) (curcumin differentially modulates mRNA profiles in Jurkat T and human peripheral blood mononuclear cells) Interferons Role: BSU (Biological study, unclassified), BIOL (Biological study) (gamma curcumin differentially modulates mRNA profiles in Jurkat T and human peripheral blood mononuclear cells) curcumin mRNA lymphocyte monocyte gene antitumor | ||||
| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 500 Naturwissenschaften und Mathematik > 540 Chemie | ||||
| Status: | Veröffentlicht | ||||
| Begutachtet: | Ja, diese Version wurde begutachtet | ||||
| An der Universität Regensburg entstanden: | Nein | ||||
| Dokumenten-ID: | 17211 |
Zusammenfassung
Curcumin, the yellow pigment of the rhizome of Curcuma longa is known to inhibit the transcription factors AP-1, Egr-1, NF-kB, c-myc and several important signaling kinases. We therefore investigated the differential effects of curcumin in concn. between 1.5 and 13.6 micro M on gene expression in T Jurkat CD4+ and human peripheral blood mononuclear cells (PBMCs). Relative quantification with ...
Zusammenfassung
Curcumin, the yellow pigment of the rhizome of Curcuma longa is known to inhibit the transcription factors AP-1, Egr-1, NF-kB, c-myc and several important signaling kinases. We therefore investigated the differential effects of curcumin in concn. between 1.5 and 13.6 micro M on gene expression in T Jurkat CD4+ and human peripheral blood mononuclear cells (PBMCs). Relative quantification with reverse transcription real-time PCR (RT-rt-PCR) showed that low concns. of curcumin significantly down-regulated mitogen-induced granulocyte macrophage colony stimulating factor (GM-CSF) mRNA (3- to 5-fold at 3 micro M) in a dose- and time-dependent manner in both cell types. In comparison, the down-regulation of inducible nitric oxide (iNOS) mRNA levels was less pronounced, but interferon gamma (IFN-gamma ) mRNA was dose-dependently up-regulated with curcumin concns. up to 8.2 micro M. Cyclin D1 mRNA expression was specifically inhibited in Jurkat T cells and stimulated PBMCs. The transcription factors NF-kB and NF-ATc were not affected in PBMCs. Interleukin-2 (IL-2), and -6 (IL-6) mRNAs levels were not influenced markedly by curcumin in stimulated PBMCs, but significantly reduced in stimulated Jurkat T cells. In addn., cytotoxic effects and down-regulation of mRNAs, including p65 and the house-keeping genes could only be measured in Jurkat T cells. These findings confirm previous reports on the anti-neoplastic potential of curcumin and show that this compd. differentially modulates the expression profile of Th1 cells and PBMCs.
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