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Rapid assignment of solution ^{31}P NMR spectra of large proteins by solid-state spectroscopy

Iuga, Adriana ; Spörner, Michael ; Ader, Christian ; Brunner, Eike ; Kalbitzer, Hans-Robert
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Date of publication of this fulltext: 05 Aug 2009 13:34


Abstract

The application of the 31P NMR spectroscopy to large proteins or protein complexes in solution is hampered by a relatively low intrinsic sensitivity coupled with large line widths. Therefore, the assignment of the phosphorus signals by two-dimensional NMR methods in solution is often extremely time consuming. In contrast, the quality of solid-state NMR spectra is not dependent on the molecular ...

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