Quantitation of mRNA for the beta-adrenoceptor gene in human mononuclear leucocytes by in situ hybridization with fluorochrome labeled cloned DNA

URN to cite this document:

urn:nbn:de:bvb:355-epub-196370 Copy

DOI to cite this document:

10.5283/epub.19637 Copy

Liebl, B. ; Pachmann, K. ; Römer, U. ; Haen, Ekkehard ; Hallek, M. ; Emmerich, B.

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Date of publication of this fulltext: 18 Feb 2011 10:23

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Details

Item type:	Article
Journal or Publication Title:	Journal of receptor research
Publisher:	Dekker
Volume:	11
Number of Issue or Book Chapter:	1-4
Page Range:	pp. 473-482
Date:	1991
Institutions:	Medicine > Lehrstuhl für Psychiatrie und Psychotherapie
Identification Number:	Value Type 1653348 PubMed ID
Classification:	Notation Type Adolescent MESH Adult MESH Cloning, Molecular MESH DNA Probes MESH Female MESH Fluorescein-5-isothiocyanate MESH Fluoresceins MESH Humans MESH Leukocytes, Mononuclear/chemistry MESH Male MESH Middle Aged MESH Nucleic Acid Hybridization MESH RNA, Messenger/genetics MESH Receptors, Adrenergic, beta/genetics MESH Thiocyanates MESH Transcription, Genetic/genetics MESH
Dewey Decimal Classification:	600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Unknown
Created at the University of Regensburg:	Unknown
Item ID:	19637

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Abstract

Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood ...

Abstract

Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood of healthy volunteers were permeabilized, fixed and attached to adhesive glass slides. After incubation with fluorochrome-labeled cloned hamster beta AR-DNA probes the amount of DNA-mRNA hybrids formed in the cytoplasm of individual cells was analyzed by microfluorimetry of specifically bound fluorochrome. In nine of ten normal subjects studied so far a fraction of beta AR-mRNA positive MNL could be detected. The proportion of positive cells as well as their signal intensity revealed a high interindividual variance.

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Metadata last modified: 26 Nov 2020 07:32

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