Liebl, B., Pachmann, K., Römer, U., Haen, Ekkehard, Hallek, M. und Emmerich, B.
(1991)
Quantitation of mRNA for the beta-adrenoceptor gene in human mononuclear leucocytes by in situ hybridization with fluorochrome labeled cloned DNA.
Journal of receptor research 11 (1-4), S. 473-482.
Veröffentlichungsdatum dieses Volltextes: 18 Feb 2011 10:23Zum PubMed-Eintrag dieses Artikels
Zusammenfassung
Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood ...
Zusammenfassung
Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood of healthy volunteers were permeabilized, fixed and attached to adhesive glass slides. After incubation with fluorochrome-labeled cloned hamster beta AR-DNA probes the amount of DNA-mRNA hybrids formed in the cytoplasm of individual cells was analyzed by microfluorimetry of specifically bound fluorochrome. In nine of ten normal subjects studied so far a fraction of beta AR-mRNA positive MNL could be detected. The proportion of positive cells as well as their signal intensity revealed a high interindividual variance.
Bibliographische Daten exportieren
| Dokumentenart: | Artikel |
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| Datum: | 1991 |
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| Institutionen: | Medizin > Lehrstuhl für Psychiatrie und Psychotherapie |
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| Identifikationsnummer: | |
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| Klassifikation: | | Notation | Art |
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| Adolescent | MESH | | Adult | MESH | | Cloning, Molecular | MESH | | DNA Probes | MESH | | Female | MESH | | Fluorescein-5-isothiocyanate | MESH | | Fluoresceins | MESH | | Humans | MESH | | Leukocytes, Mononuclear/chemistry | MESH | | Male | MESH | | Middle Aged | MESH | | Nucleic Acid Hybridization | MESH | | RNA, Messenger/genetics | MESH | | Receptors, Adrenergic, beta/genetics | MESH | | Thiocyanates | MESH | | Transcription, Genetic/genetics | MESH |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Unbekannt / Keine Angabe |
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| An der Universität Regensburg entstanden: | Unbekannt / Keine Angabe |
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| Dokumenten-ID: | 19637 |
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