Liebl, B., Pachmann, K., Römer, U., Haen, Ekkehard, Hallek, M. and Emmerich, B.
(1991)
Quantitation of mRNA for the beta-adrenoceptor gene in human mononuclear leucocytes by in situ hybridization with fluorochrome labeled cloned DNA.
Journal of receptor research 11 (1-4), pp. 473-482.
Date of publication of this fulltext: 18 Feb 2011 10:23at PubMed
Abstract
Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood ...
Abstract
Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood of healthy volunteers were permeabilized, fixed and attached to adhesive glass slides. After incubation with fluorochrome-labeled cloned hamster beta AR-DNA probes the amount of DNA-mRNA hybrids formed in the cytoplasm of individual cells was analyzed by microfluorimetry of specifically bound fluorochrome. In nine of ten normal subjects studied so far a fraction of beta AR-mRNA positive MNL could be detected. The proportion of positive cells as well as their signal intensity revealed a high interindividual variance.
Export bibliographical data
| Item type: | Article |
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| Date: | 1991 |
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| Institutions: | Medicine > Lehrstuhl für Psychiatrie und Psychotherapie |
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| Identification Number: | | Value | Type |
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| 1653348 | PubMed ID |
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| Classification: | | Notation | Type |
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| Adolescent | MESH | | Adult | MESH | | Cloning, Molecular | MESH | | DNA Probes | MESH | | Female | MESH | | Fluorescein-5-isothiocyanate | MESH | | Fluoresceins | MESH | | Humans | MESH | | Leukocytes, Mononuclear/chemistry | MESH | | Male | MESH | | Middle Aged | MESH | | Nucleic Acid Hybridization | MESH | | RNA, Messenger/genetics | MESH | | Receptors, Adrenergic, beta/genetics | MESH | | Thiocyanates | MESH | | Transcription, Genetic/genetics | MESH |
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| Dewey Decimal Classification: | 600 Technology > 610 Medical sciences Medicine |
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| Status: | Published |
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| Refereed: | Unknown |
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| Created at the University of Regensburg: | Unknown |
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| Item ID: | 19637 |
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