Quantitation of mRNA for the beta-adrenoceptor gene in human mononuclear leucocytes by in situ hybridization with fluorochrome labeled cloned DNA

URN to cite this document:: urn:nbn:de:bvb:355-epub-196370
DOI to cite this document:: 10.5283/epub.19637

Liebl, B. ; Pachmann, K. ; Römer, U. ; Haen, Ekkehard ; Hallek, M. ; Emmerich, B.

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Date of publication of this fulltext: 18 Feb 2011 10:23

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Abstract

Determination of the transcriptional activity of the beta-adrenoceptor (beta-AR) gene in human mononuclear leucocytes (MNL) may provide important information concerning variations in receptor expression in clinical studies. We used a new method with highly fluorochrome-labeled gene probes for in situ hybridization which allows a rapid detection of mRNA at the cellular level. MNL from venous blood ...