Novel multicolor fluorescently labeled silica nanoparticles for interface fluorescence resonance energy transfer to and from labeled avidin

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.19982

Saleh, Sayed M. ; Müller, Rainer ; Mader, Heike S. ; Dürkop, Axel ; Wolfbeis, Otto S.

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Veröffentlichungsdatum dieses Volltextes: 18 Mrz 2011 06:52

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Zusammenfassung

Fluorescent silica nanoparticles (SiNPs) were prepared by covalent attachment of fluorophores to the amino-modified surface of SiNPs with a typical diameter of 15 nm. The SiNPs are intended for use in novel kinds of fluorescence resonance energy transfer (FRET)-based affinity assays at the interface between nanoparticle and sample solution. Various labels were employed to obtain a complete set of ...