New diode laser-excitable green fluorescent label and its application to detection of bovine serum albumin via microchip electrophoresis

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.20010

Link, Martin ; Schulze, Philipp ; Belder, Detlev ; Wolfbeis, Otto S.

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Veröffentlichungsdatum dieses Volltextes: 18 Mrz 2011 07:03

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Zusammenfassung

A novel amino-reactive fluorescent label is presented that is based on a yellow daylight chromophore and fluorophore. Its absorption band is wide and peaks at 431 nm in water solution, thus well matching the lines of either the 375-nm and the 431-nm diode lasers and of many frequency-variable dye lasers. When conjugated to bovine serum albumin (BSA), the fluorescence peaks at 501 nm with a ...