Comparison of a Nucleosidic vs Non-Nucleosidic Postsynthetic “Click” Modification of DNA with Base-Labile Fluorescent Probes

URN to cite this document:: urn:nbn:de:bvb:355-epub-200430
DOI to cite this document:: 10.5283/epub.20043

Berndl, Sina ; Herzig, Nadine ; Kele, Péter ; Lachmann, Daniel ; Li, Xiaohua ; Wolfbeis, Otto S.

; Wagenknecht, Hans-Achim

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Date of publication of this fulltext: 18 Mar 2011 07:12

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Abstract

The azides 1 and 2 bearing a phenoxazinium and a coumarin fluorophore, respectively, were applied in postsynthetic "click"-type bioconjugation and coupled to oligonucleotides modified with alkyne groups using two alternative approaches: (i) as a nucleotide modification at the 2'-position of uridine and (ii) as a nucleotide substitution using (S)-(-)-3-amino-l,2-propanediol as an acyclic linker ...