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Comparison of a Nucleosidic vs Non-Nucleosidic Postsynthetic “Click” Modification of DNA with Base-Labile Fluorescent Probes

URN to cite this document:
Berndl, Sina ; Herzig, Nadine ; Kele, Péter ; Lachmann, Daniel ; Li, Xiaohua ; Wolfbeis, Otto S. ; Wagenknecht, Hans-Achim
Date of publication of this fulltext: 18 Mar 2011 07:12


The azides 1 and 2 bearing a phenoxazinium and a coumarin fluorophore, respectively, were applied in postsynthetic “click”-type bioconjugation and coupled to oligonucleotides modified with alkyne groups using two alternative approaches: (i) as a nucleotide modification at the 2′-position of uridine and (ii) as a nucleotide substitution using (S)-(−)-3-amino-1,2-propanediol as an acyclic linker ...


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