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Method for simultaneous luminescence sensing of two species using optical probes of different decay time, and its application to an enzymatic reaction at varying temperature

DOI to cite this document:
Nagl, Stefan ; Stich, Matthias I. J. ; Schäferling, Michael ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 07:14


Chemical sensing, imaging and microscopy based on the use of fluorescent probes has so far been limited almost exclusively to the detection of a single parameter at a time. We present a scheme that can overcome this limitation by enabling optical sensing of two parameter simultaneously and even at identical excitation and emission wavelengths of two probes provided (a) their decay times are ...


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