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Method for simultaneous luminescence sensing of two species using optical probes of different decay time, and its application to an enzymatic reaction at varying temperature

Nagl, Stefan, Stich, Matthias I. J., Schäferling, Michael and Wolfbeis, Otto S. (2009) Method for simultaneous luminescence sensing of two species using optical probes of different decay time, and its application to an enzymatic reaction at varying temperature. Analytical and Bioanalytical Chemistry 393 (4), pp. 1199-1207.

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Abstract

Chemical sensing, imaging and microscopy based on the use of fluorescent probes has so far been limited almost exclusively to the detection of a single parameter at a time. We present a scheme that can overcome this limitation by enabling optical sensing of two parameter simultaneously and even at identical excitation and emission wavelengths of two probes provided (a) their decay times are ...

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Item type:Article
Date:2009
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1007/s00216-008-2467-0DOI
Keywords:Dual sensing - Luminescence lifetime - Oxygen sensing - Temperature sensing - Fluorescence imaging
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20046
Owner only: item control page

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