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Dual Labeling of Biomolecules by Using Click Chemistry: A Sequential Approach

Kele, Péter ; Mezö, Gábor ; Achatz, Daniela ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 07:16


Abstract

Click by click: Dual labeling of model compounds was carried out by using copper-free and copper-mediated click chemistry in a sequential manner. This method was used to introduce two labels onto biological targets or nanoparticles, thus quickly converting them into fluorescence resonance energy transfer systems.


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