Fully reversible optical biosensors for uric acid using oxygen transduction

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.20050

Schrenkhammer, Petra ; Wolfbeis, Otto S.

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Veröffentlichungsdatum dieses Volltextes: 18 Mrz 2011 07:26

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Zusammenfassung

An optical biosensor is presented for continuous determination of uric acid. The scheme is based on the measurement of the consumption of oxygen during the oxidation of uric acid that is catalyzed by the enzyme uricase. The enzyme is immobilized in a polyurethane hydrogel next to a metal-organic probe whose fluorescence is quenched by oxygen. The consumption of oxygen was followed by measurement ...