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- DOI zum Zitieren dieses Dokuments:
- 10.5283/epub.20093
Alternative Links zum Volltext:DOI
Zusammenfassung
A single-step end point method is presented for determination of the activity of the enzyme alkaline phosphatase (ALP) using the effect of enhancement of fluorescence of the easily accessible europium(III)-tetracycline 3:1 complex (Eu3TC). Its luminescence, peaking at 616 nm if excited at 405 nm, is enhanced by a factor of 2.5 in the presence of phosphate. Phenyl phosphate was used as a substrate ...
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