A Resonance Energy Transfer Immunoassay Based on a Thiol-Reactive Ruthenium Donor Dye and a Longwave-Emitting Acceptor

DOI to cite this document:: 10.5283/epub.20199

Weh, Jochen ; Dürkop, Axel ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 18 Mar 2011 08:03

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Abstract

A novel immunoassay is described that applies a thiol-reactive ruthenium metal–ligand complex as the donor dye in a luminescence energy transfer (LET) detection scheme. Unlike amine-reactive labels, the LET with a thiol label allows improved specificity and better reproducibility of labelling positions on proteins, because the number of reactive thiol groups of proteins is distinctly smaller. ...