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A Resonance Energy Transfer Immunoassay Based on a Thiol-Reactive Ruthenium Donor Dye and a Longwave-Emitting Acceptor

Weh, Jochen, Dürkop, Axel and Wolfbeis, Otto S. (2007) A Resonance Energy Transfer Immunoassay Based on a Thiol-Reactive Ruthenium Donor Dye and a Longwave-Emitting Acceptor. ChemBioChem: a European journal of chemical biology 8 (1), pp. 122-128.

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Abstract

A novel immunoassay is described that applies a thiol-reactive ruthenium metal–ligand complex as the donor dye in a luminescence energy transfer (LET) detection scheme. Unlike amine-reactive labels, the LET with a thiol label allows improved specificity and better reproducibility of labelling positions on proteins, because the number of reactive thiol groups of proteins is distinctly smaller. ...

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Item type:Article
Date:2 January 2007
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1002/cbic.200600316DOI
Keywords:FRET; immunoassays; luminescence; ruthenium metal–ligand complex; thiol label
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20199
Owner only: item control page

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