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Sensitive luminescent determination of DNA using the terbium(III)–difloxacin complex

Yegorova, Alla V. ; Scripinets, Yulia V. ; Dürkop, Axel ; Karasyov, Alexander A. ; Antonovich, Valery P. ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 18 Mar 2011 08:05


Abstract

The interaction of the terbium–difloxacin complex (Tb–DFX) with DNA has been examined by using UV–vis absorption and luminescence spectroscopy. The Tb–DFX complex shows an up to 85-fold enhancement of luminescence intensity upon titration with DNA. The long decay times allow additional detection schemes like time-resolved measurements in microplate readers to enhance sensitivity by off-gating ...

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