A Novel Method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.20295

Lin, Zhihong ; Wu, Meng ; Wolfbeis, Otto S. ; Schäferling, Michael

PDF
(284kB) - Nur für Mitarbeiter des Archivs

Veröffentlichungsdatum dieses Volltextes: 04 Apr 2011 10:29

Alternative Links zum Volltext:DOI

Details

Bibliographische Daten exportieren

Zusammenfassung

A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H2O2 (HP), using a fluorescent europium–tetracycline (Eu3TC) complex as indicator. On addition of HP, this complex forms a highly fluorescent adduct (Eu3TC–HP), which is decomposed in the presence of POx to form the weakly ...