Lin, Zhihong, Wu, Meng, Wolfbeis, Otto S. and Schäferling, Michael (2006) A Novel Method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay. Chemistry: a European journal 12 (10), pp. 2730-2738.
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Abstract
A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H2O2 (HP), using a fluorescent europium–tetracycline (Eu3TC) complex as indicator. On addition of HP, this complex forms a highly fluorescent adduct (Eu3TC–HP), which is decomposed in the presence of POx to form the weakly ...
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| Item type: | Article | ||||
|---|---|---|---|---|---|
| Date: | 20 March 2006 | ||||
| Institutions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) | ||||
| Identification Number: |
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| Keywords: | fluorescent probes; imaging agents; immunoassays; peroxidase activity; time-resolved spectrometry | ||||
| Dewey Decimal Classification: | 500 Science > 540 Chemistry & allied sciences | ||||
| Status: | Published | ||||
| Refereed: | Unknown | ||||
| Created at the University of Regensburg: | Unknown | ||||
| Item ID: | 20295 |
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