A Novel Method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay

DOI to cite this document:: 10.5283/epub.20295

Lin, Zhihong ; Wu, Meng ; Wolfbeis, Otto S.

; Schäferling, Michael

PDF
(284kB) - Repository staff only

Date of publication of this fulltext: 04 Apr 2011 10:29

Alternative links to fulltext:DOI

Details

Indexed in

Export bibliographical data

Abstract

A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H2O2 (HP), using a fluorescent europium-tetracycline (Eu3TC) complex as indicator. On addition of HP, this complex forms a highly fluorescent adduct (Eu3TC-HP), which is decomposed in the presence of POx to form the weakly ...