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A Novel Method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay

Lin, Zhihong ; Wu, Meng ; Wolfbeis, Otto S. ; Schäferling, Michael
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Date of publication of this fulltext: 04 Apr 2011 10:29


Abstract

A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H2O2 (HP), using a fluorescent europium–tetracycline (Eu3TC) complex as indicator. On addition of HP, this complex forms a highly fluorescent adduct (Eu3TC–HP), which is decomposed in the presence of POx to form the weakly ...

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