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A Novel Method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay

Lin, Zhihong, Wu, Meng, Wolfbeis, Otto S. and Schäferling, Michael (2006) A Novel Method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of Peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay. Chemistry: a European journal 12 (10), pp. 2730-2738.

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Abstract

A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H2O2 (HP), using a fluorescent europium–tetracycline (Eu3TC) complex as indicator. On addition of HP, this complex forms a highly fluorescent adduct (Eu3TC–HP), which is decomposed in the presence of POx to form the weakly ...

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Item type:Article
Date:20 March 2006
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1002/chem.200500884DOI
Keywords:fluorescent probes; imaging agents; immunoassays; peroxidase activity; time-resolved spectrometry
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20295
Owner only: item control page

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