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Screening Scheme Based on Measurement of Fluorescence Lifetime in the Nanosecond Domain

Höfelschweiger, Bianca K. ; Pfeifer, Lutz ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 04 Apr 2011 10:42


Abstract

The authors demonstrate that the fluorescence lifetime of certain fluorescent labels is a useful parameter to detect affinity binding between biotin and streptavidin, as well as between biotinylated bovine serum albumin and streptavidin. The assay is performed in a microplate format, and lifetimes are determined using dye laser-induced fluorescence. Four fluorescent labels are presented that ...

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