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Screening Scheme Based on Measurement of Fluorescence Lifetime in the Nanosecond Domain

Höfelschweiger, Bianca K., Pfeifer, Lutz and Wolfbeis, Otto S. (2005) Screening Scheme Based on Measurement of Fluorescence Lifetime in the Nanosecond Domain. Journal of Biomolecular Screening 10 (7), pp. 687-694.

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The authors demonstrate that the fluorescence lifetime of certain fluorescent labels is a useful parameter to detect affinity binding between biotin and streptavidin, as well as between biotinylated bovine serum albumin and streptavidin. The assay is performed in a microplate format, and lifetimes are determined using dye laser-induced fluorescence. Four fluorescent labels are presented that ...


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Item type:Article
Date:October 2005
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
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Keywords:Laser-induced fluorescence; lifetime; fluorescent label; affinity binding; affinity assay; decay time
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Created at the University of Regensburg:Unknown
Item ID:20359
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