Go to content
UR Home

Screening Scheme Based on Measurement of Fluorescence Lifetime in the Nanosecond Domain

Höfelschweiger, Bianca K., Pfeifer, Lutz and Wolfbeis, Otto S. (2005) Screening Scheme Based on Measurement of Fluorescence Lifetime in the Nanosecond Domain. Journal of Biomolecular Screening 10 (7), pp. 687-694.

[img]PDF
Download (142kB) - Repository staff only
Date of publication of this fulltext: 04 Apr 2011 10:42

at publisher (via DOI)


Abstract

The authors demonstrate that the fluorescence lifetime of certain fluorescent labels is a useful parameter to detect affinity binding between biotin and streptavidin, as well as between biotinylated bovine serum albumin and streptavidin. The assay is performed in a microplate format, and lifetimes are determined using dye laser-induced fluorescence. Four fluorescent labels are presented that ...

plus


Export bibliographical data



Item type:Article
Date:October 2005
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Identification Number:
ValueType
10.1177/1087057105277493DOI
Keywords:Laser-induced fluorescence; lifetime; fluorescent label; affinity binding; affinity assay; decay time
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Unknown
Created at the University of Regensburg:Unknown
Item ID:20359
Owner only: item control page

Downloads

Downloads per month over past year

  1. Homepage UR

University Library

Publication Server

Contact:

Publishing: oa@ur.de

Dissertations: dissertationen@ur.de

Research data: daten@ur.de

Contact persons