Time-resolved enzymatic determination of glucose using a fluorescent europium probe for hydrogen peroxide

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.20370

Wu, Meng ; Lin, Zhihong ; Dürkop, Axel ; Wolfbeis, Otto S.

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Veröffentlichungsdatum dieses Volltextes: 04 Apr 2011 10:51

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An enzymatic assay for glucose based on the use of the fluorescent probe for hydrogen peroxide, europium(III) tetracycline (EuTc), is described. The weakly fluorescent EuTc and enzymatically generated H2O2 form a strongly fluorescent complex (EuTc–H2O2) whose fluorescence decay profile is significantly different. Since the decay time of EuTc–H2O2 is in the microseconds time domain, fluorescence ...