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Chameleon Labels for Staining and Quantifying Proteins

Wetzl, Bianca K., Yarmoluk, Sergiy M., Craig, Douglas B. and Wolfbeis, Otto S. (2004) Chameleon Labels for Staining and Quantifying Proteins. Angewandte Chemie International Edition 43 (40), pp. 5400-5402.

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Glowing marks: A new class of protein stains, the pyrylium dyes, undergo a strong color change (typically from blue to red, see picture) on covalently binding to proteins. While the free stains are almost nonfluorescent, the protein-conjugated forms are highly fluorescent. The dyes do not alter the charge of a protein, and thus do not change its electrophoretic properties. The stains also can be used in quantitative protein assays.

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Item type:Article
Date:11 October 2004
Institutions:Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
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Keywords:electrophoresis;fluorescent probes;protein assay;proteomics
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
Created at the University of Regensburg:Unknown
Item ID:20383
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