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Chameleon Labels for Staining and Quantifying Proteins

Wetzl, Bianca K. ; Yarmoluk, Sergiy M. ; Craig, Douglas B. ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 06 Apr 2011 09:16


Abstract

Glowing marks: A new class of protein stains, the pyrylium dyes, undergo a strong color change (typically from blue to red, see picture) on covalently binding to proteins. While the free stains are almost nonfluorescent, the protein-conjugated forms are highly fluorescent. The dyes do not alter the charge of a protein, and thus do not change its electrophoretic properties. The stains also can be used in quantitative protein assays.


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