Fluorescent pH sensors with negligible sensitivity to ionic strength

DOI to cite this document:: 10.5283/epub.20387

Weidgans, Bernhard M. ; Krause, Christian ; Klimant, Ingo ; Wolfbeis, Otto S.

PDF
(139kB) - Repository staff only

Date of publication of this fulltext: 15 Apr 2011 08:40

Alternative links to fulltext:DOI

Details

Export bibliographical data

Abstract

Optical pH determination has the fundamental disadvantage of measuring a signal that depends on the ionic strength of the sample. The problem originates from the complex relationship between the proton activity and the concentration of the pH-sensitive dye. The effect of ionic strength on the signal depends on the charge of the indicator and its environment, e.g. the immobilisation matrix. We ...