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Fluorescent pH sensors with negligible sensitivity to ionic strength

Weidgans, Bernhard M. ; Krause, Christian ; Klimant, Ingo ; Wolfbeis, Otto S.
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Date of publication of this fulltext: 15 Apr 2011 08:40


Abstract

Optical pH determination has the fundamental disadvantage of measuring a signal that depends on the ionic strength of the sample. The problem originates from the complex relationship between the proton activity and the concentration of the pH-sensitive dye. The effect of ionic strength on the signal depends on the charge of the indicator and its environment, e.g. the immobilisation matrix. We ...

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