Assessment of major histocompatibility complex class I interaction with Epstein-Barr virus and human immunodeficiency virus peptides by elevation of membrane H-2 and HLA in peptide loading-deficient cells

URN zum Zitieren dieses Dokuments:

urn:nbn:de:bvb:355-epub-204132 Copy

DOI zum Zitieren dieses Dokuments:

10.5283/epub.20413 Copy

Stuber, G. ; Modrow, Susanne ; Höglund, P. ; Franksson, L. ; Elvin, J. ; Wolf, Hans J. ; Kärre, K. ; Klein, G.

Vorschau

PDF (733kB)

Veröffentlichungsdatum dieses Volltextes: 12 Apr 2011 07:23

---

Alternative Links zum Volltext:PubmedDOI

---

Details

Dokumentenart:	Artikel
Titel eines Journals oder einer Zeitschrift:	European journal of immunology
Verlag:	Wiley-VCH
Band:	22
Nummer des Zeitschriftenheftes oder des Kapitels:	10
Seitenbereich:	S. 2697-2703
Datum:	1992
Institutionen:	Medizin > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Identifikationsnummer:	Wert Typ 1327802 PubMed-ID 10.1002/eji.1830221033 DOI
Klassifikation:	Notation Art Amino Acid Sequence MESH Animals MESH Antigens, Viral/immunology MESH DNA-Binding Proteins/immunology MESH Epstein-Barr Virus Nuclear Antigens MESH Gene Products, gag/immunology MESH H-2 Antigens/analysis MESH HIV-1/immunology MESH HLA-A2 Antigen/analysis MESH Herpesvirus 4, Human/immunology MESH Humans MESH Mice MESH Mice, Inbred C57BL MESH Molecular Sequence Data MESH Viral Proteins/immunology MESH
Dewey-Dezimal-Klassifikation:	600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin
Status:	Veröffentlicht
Begutachtet:	Unbekannt / Keine Angabe
An der Universität Regensburg entstanden:	Unbekannt / Keine Angabe
Dokumenten-ID:	20413

Vorschau

Bibliographische Daten exportieren

ASCII CitationAtomBibTeXData Cite XMLDataCite XML OAIDublin CoreDublin Core (LZV)EndNoteHTML CitationJSONJSON LDMETSMODSOPENAIREOpenAPCRDF+N-TriplesRDF+N3RDF+XMLReferReference ManagerSimple MetadataXMLopenCostxMetaDissPlus

---

Zusammenfassung

Earlier findings indicate that peptides can affect the expression of major histocompatibility complex (MHC) class I molecules on the surface of cells with defective peptide loading mechanism. We have used peptide induced increase of class I antigen expression to assess peptide interaction with MHC class I molecules. A panel of 41 overlapping synthetic peptides derived from the human ...

Zusammenfassung

Earlier findings indicate that peptides can affect the expression of major histocompatibility complex (MHC) class I molecules on the surface of cells with defective peptide loading mechanism. We have used peptide induced increase of class I antigen expression to assess peptide interaction with MHC class I molecules. A panel of 41 overlapping synthetic peptides derived from the human immunodeficiency virus-1 (HIV-1) gag protein and 33 nonoverlapping peptides from Epstein-Barr virus (EBV) proteins EBNA-1, 2, 3, 4, 5, 6, LMP, BZLF2, BILF2, BSLF2, BALF4 and BcLF1 was assessed for the ability to enhance the expression of HLA-A2.1, H-2Db, Kb and Dd on the murine RMA-S and human 721.174/T2 (.174/T2) lines by indirect immunofluorescence. Considering doubling of the fluorescence intensity in the peptide-treated samples as positivity, 6 of 39 HIV and 1 of 32 EBV peptides were found to bind to A2.1, 6 of 39 HIV gag and 7 of 16 EBV peptides to Db, 8 of 39 HIV gag and 5 of 16 EBV peptides to Kb and 2 of 39 HIV gag and 1 of 17 EBV peptides to Dd. The sensitivity of the method is comparable to the in vitro class I assembly assay with conformation-dependent monoclonal antibody and is more discriminating than the solid-phase assay. Due to its simplicity this method can also serve for testing large peptide panels for binding capacity to various class I molecules. Moreover, the method provides information about the relevance of in vitro tests for class I assembly in living cells.

---

---

Metadaten zuletzt geändert: 29 Sep 2021 07:38

Nur für Besitzer und Autoren: Kontrollseite des Eintrags

Downloadstatistik

Downloadstatistik

Downloadstatistik

Downloadstatistik

Downloads

Downloads im Monat während des letzten Jahres

Altmetric

Altmetric

Altmetric

Alternative Statistik (altmetrics)

Weitere Literatur (mittels CORE)

Weitere Literatur (mittels CORE)

Weitere Literatur (mittels CORE)

Weitere Literatur (mittels CORE)