Homogeneous Luminescence Decay Time-Based Assay Using Energy Transfer from Nanospheres

DOI zum Zitieren dieses Dokuments:: 10.5283/epub.20445

Kürner, Jens M. ; Wolfbeis, Otto S. ; Klimant, Ingo

PDF
(185kB) - Nur für Mitarbeiter des Archivs

Veröffentlichungsdatum dieses Volltextes: 15 Apr 2011 09:16

Alternative Links zum Volltext:DOI

Details

Bibliographische Daten exportieren

Zusammenfassung

Following a study on the feasibility of resonance energy transfer (RET) from carboxylated nanospheres with an incorporated phosphorescent donor to a cationic polyelectrolyte/acceptor aggregate on their surface, a novel scheme for homogeneous assays is presented that is based on RET from phosphorescent biotinylated nanospheres to fluorescently labeled streptavidin (SA). The phosphorescent ...