Fluorescent Imaging of pH with Optical Sensors Using Time Domain Dual Lifetime Referencing

DOI to cite this document:: 10.5283/epub.20446

Liebsch, Gregor ; Klimant, Ingo ; Krause, Christian ; Wolfbeis, Otto S.

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Date of publication of this fulltext: 15 Apr 2011 09:17

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Abstract

We present a referenced scheme for fluorescence intensity measurements that is useful for imaging applications. It is based on the conversion of the fluorescence intensity information into a time-dependent parameter. A phosphorescent dye is added in the form of 10-μm particles to the sample containing the pH-sensitive fluorescent indicator. Both the reference dye and the pH probe are excited ...